Isoenzyme Expression of seeds and seedlings of red rice under the action of the extract of two species Araceae

Authors

  • Tiago Zanatta Aumonde UFPEL
  • Tiago Pedó UFPEL
  • Emanuela Garbin Martinazzo UFPEL
  • Junior Borella UFPEL
  • Francisco Amaral Villela UFPEL

Abstract

Several plant extracts have potential fitoxicidez seeds and sprouts , so the assessment of isoenzymes is an important tool in assessing the quality of the seed . The aim of this study was to evaluate the effect of extracts of two species of the family Araceae ( Philodendron bipinnatifidum and Zantedeschia aethiopica ) on the expression of isozymes of seeds and seedlings of red rice . The concentrations of the extracts were 0, 12, 25, 50 and 75%. Were evaluated in stages : seed, first count and germination test . The expression of esterase isoenzymes , acid phosphatase , and glutamate oxaloacetate transaminase peroxidase was determined by vertical electrophoresis system polyacrylamide gel and interpretation of the results was by visual analysis of the gels . The extracts of the species of the family Araceae altered isozyme expression , and the intensity and number of bands with variable concentration and the stage of evaluation.

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Author Biographies

  • Tiago Zanatta Aumonde, UFPEL
    Agr. MSc., PhD in S & T Seeds, CAPES Scholarship, UFPEL
  • Tiago Pedó, UFPEL
    Agr. MSc., PhD in S & T Seeds, CAPES Scholarship, UFPEL
  • Emanuela Garbin Martinazzo, UFPEL
    Biologist, Dr., Post-Doctoral PNPD CAPES, Plant Physiology, UFPEL
  • Junior Borella, UFPEL
    Biologist, PhD in Plant Physiology. Bolssita CAPES, UFPel
  • Francisco Amaral Villela, UFPEL
    Prof. Dr.. the Graduate Program in C & T Seeds, Fellow Research Productivity CNPq, UFPEL

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Published

2013-08-22

Issue

Vol. 56 No. 3 (2013): RCA

Section

Scientific Note

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